Cloning and characterization of the white gene from Anopheles gambiae
Identifieur interne : 004205 ( Main/Exploration ); précédent : 004204; suivant : 004206Cloning and characterization of the white gene from Anopheles gambiae
Auteurs : N. J. Besansky [Géorgie (pays), États-Unis] ; J. A. Bedell [Géorgie (pays), États-Unis] ; M. Q. Benedict [Géorgie (pays), États-Unis] ; O. Mukabayire [Géorgie (pays), États-Unis] ; D. Hilfiker [États-Unis] ; F. H. Collins [Géorgie (pays), États-Unis]Source :
- Insect Molecular Biology [ 0962-1075 ] ; 1995-11.
English descriptors
- Teeft :
- Allele, Allelic mutations, Amino terminus, Anopheles, Anopheles albimanus, Anopheles gambiae, Besansky, Cdna, Cdna clone, Cdna clones, Cdna libraries, Cdna library, Cherbas cherbas, Chromosome, Clone, Coding region, Coding regions, Codon, Codon usage, Cold spring harbor laboratory press, Colour, Colour gene, Colour genes, Colour mutants, Colour mutations, Dipteran species, Disease control, Drosophila, Drosophila genes, Drosophila melanogaster, Effective number, Exon, Fruitfly, Fruitfly gene, Gambiae, Gambiae gene, Gambiae genes, Gambiae genomic, Gene, Gene expression, Genetics computer group, Genomic, Genomic clones, Genomic sequence, Genomic sequences, Insertion, Intron, Locus, London school, Melanogaster, Microsatellite mapping, Molec, Mosquito, Mosquito anopheles gambiae, Mrna, Mutant, Mutant strains, Mutation, Northern blot analysis, Northern blots, Nucleic acids, Nucleotide sequence, Perkin elmer, Phenol extractions, Pigment precursors, Pirotta brockl, Polyadenylation, Polyadenylation signal, Polyadenylation site, Primer, Restriction maps, Small transposable element, Strand primer, Strand primers, Tropical medicine, Unpublished data, Variable sites, West africa, White alleles, White expression, White eyes, White gene, White genes, White locus, White protein.
Abstract
A 14 kb region of genomic DN A containing the X‐linked Anopheles gambiae eye colour gene, white, was cloned and sequenced. Genomic clones containing distinct white+ alleles were polymorphic for the insertion of a small transposable element in intron 3, and differed at 1% of nucleotide positions compared. Sequence was also determined from a rare 2914 bp cDNA. Comparison of cDNA and genomic sequences established an intron‐exon structure distinct from Drosophila white. Despite a common trend in Anopheles and Drosophila of weak codon bias given low levels of gene expression, codon usage by Anopheles gambiae white was strongly biased. Overall amino acid identity between the predicted mosquito and fruitfly proteins was 64%, but dropped to 14% at the amino terminus. To correlate phenotypically white‐eyed strains of A. gambiae with structural lesions in white, five available strains were analysed by PCR and Southern blotting. Although these strains carried allelic mutations, independently generated by gamma radiation (three strains) or spontaneous events (two strains), no white lesions were detected. Significantly, another non‐allelic X‐linked mutation, causing an identical white‐eyed phenotype, has been correlated with a structural defect in the cloned white gene (Benedict et al., 1995). Taken together, these observations suggest that the white‐eyed mutants analysed in the present study carry mutations in a second eye colour gene and are most likely white+.
Url:
DOI: 10.1111/j.1365-2583.1995.tb00027.x
Affiliations:
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<term>Allelic mutations</term>
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<term>Anopheles</term>
<term>Anopheles albimanus</term>
<term>Anopheles gambiae</term>
<term>Besansky</term>
<term>Cdna</term>
<term>Cdna clone</term>
<term>Cdna clones</term>
<term>Cdna libraries</term>
<term>Cdna library</term>
<term>Cherbas cherbas</term>
<term>Chromosome</term>
<term>Clone</term>
<term>Coding region</term>
<term>Coding regions</term>
<term>Codon</term>
<term>Codon usage</term>
<term>Cold spring harbor laboratory press</term>
<term>Colour</term>
<term>Colour gene</term>
<term>Colour genes</term>
<term>Colour mutants</term>
<term>Colour mutations</term>
<term>Dipteran species</term>
<term>Disease control</term>
<term>Drosophila</term>
<term>Drosophila genes</term>
<term>Drosophila melanogaster</term>
<term>Effective number</term>
<term>Exon</term>
<term>Fruitfly</term>
<term>Fruitfly gene</term>
<term>Gambiae</term>
<term>Gambiae gene</term>
<term>Gambiae genes</term>
<term>Gambiae genomic</term>
<term>Gene</term>
<term>Gene expression</term>
<term>Genetics computer group</term>
<term>Genomic</term>
<term>Genomic clones</term>
<term>Genomic sequence</term>
<term>Genomic sequences</term>
<term>Insertion</term>
<term>Intron</term>
<term>Locus</term>
<term>London school</term>
<term>Melanogaster</term>
<term>Microsatellite mapping</term>
<term>Molec</term>
<term>Mosquito</term>
<term>Mosquito anopheles gambiae</term>
<term>Mrna</term>
<term>Mutant</term>
<term>Mutant strains</term>
<term>Mutation</term>
<term>Northern blot analysis</term>
<term>Northern blots</term>
<term>Nucleic acids</term>
<term>Nucleotide sequence</term>
<term>Perkin elmer</term>
<term>Phenol extractions</term>
<term>Pigment precursors</term>
<term>Pirotta brockl</term>
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<term>Polyadenylation signal</term>
<term>Polyadenylation site</term>
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<term>Restriction maps</term>
<term>Small transposable element</term>
<term>Strand primer</term>
<term>Strand primers</term>
<term>Tropical medicine</term>
<term>Unpublished data</term>
<term>Variable sites</term>
<term>West africa</term>
<term>White alleles</term>
<term>White expression</term>
<term>White eyes</term>
<term>White gene</term>
<term>White genes</term>
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<front><div type="abstract" xml:lang="en">A 14 kb region of genomic DN A containing the X‐linked Anopheles gambiae eye colour gene, white, was cloned and sequenced. Genomic clones containing distinct white+ alleles were polymorphic for the insertion of a small transposable element in intron 3, and differed at 1% of nucleotide positions compared. Sequence was also determined from a rare 2914 bp cDNA. Comparison of cDNA and genomic sequences established an intron‐exon structure distinct from Drosophila white. Despite a common trend in Anopheles and Drosophila of weak codon bias given low levels of gene expression, codon usage by Anopheles gambiae white was strongly biased. Overall amino acid identity between the predicted mosquito and fruitfly proteins was 64%, but dropped to 14% at the amino terminus. To correlate phenotypically white‐eyed strains of A. gambiae with structural lesions in white, five available strains were analysed by PCR and Southern blotting. Although these strains carried allelic mutations, independently generated by gamma radiation (three strains) or spontaneous events (two strains), no white lesions were detected. Significantly, another non‐allelic X‐linked mutation, causing an identical white‐eyed phenotype, has been correlated with a structural defect in the cloned white gene (Benedict et al., 1995). Taken together, these observations suggest that the white‐eyed mutants analysed in the present study carry mutations in a second eye colour gene and are most likely white+.</div>
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